microscope slide power meter sensor head s170c  (Thorlabs)

Journal: bioRxiv

Article Title: SHRIMP: Genetically Encoded mScarlet-derived Red Fluorescent Hydrogen Peroxide Sensor with High Brightness and Minimal Photoactivation

doi: 10.1101/2023.08.09.552302

Figure Lengend Snippet: (A) Comparison of the fluorescence brightness of HEK 293T cells at 24 h post transient transfection. Left: Representative fluorescence images of the cells expressing SHRIMP or HyPerRed. The images were acquired under similar conditions and presented in the same intensity scale. Middle: Flow cytometry analysis of HEK 293T cells expressing SHRIMP or HyPerRed. Right: Bar graph showing the average of individual cell fluorescence brightness from the flow cytometry experiment. The error bar represents s.d. and the statistical comparison was made with an unpaired, two-tailed t-test (**** P <0.0001). (B) Fluorescence intensity traces of HyPerRed (left) and SHRIMP (right) proteins encapsulated in mineral oil droplets in response to multiple cycles of five consecutive 532 nm laser scans followed by one 405 nm laser scan on a confocal fluorescence microscope. Representative data from three independent technical repeats are presented.

Article Snippet: The optical power was measured using a digital optical power meter (Thorlabs, #PM100D) with a microscope slide power meter sensor (Thorlabs, #S170C).

Techniques: Comparison, Fluorescence, Transfection, Expressing, Flow Cytometry, Two Tailed Test, Microscopy